The Kirchdoerfer lab at the University of Wisconsin-Madison uses a combination of biochemistry and structural biology to study viral proteins with a special focus on coronaviruses. Our expertise ranges from protein expression and purification to high-resolution structure determination using single-particle cryo-electron microscopy. We are particularly interested in multi-subunit viral complexes and their interactions with substrates and small molecules as well as expanding structural biology knowledge beyond SARS-CoV-2. As part of the READDI-AC consortium, we are using our lab’s expertise in viral polymerases and working closely with the multidisciplinary team in Core C to establish primary and secondary assays for viral protein function as well as providing single-particle cryoEM structure information for discovered compounds bound to their viral targets.
