The Dulin lab at the Vrije Universiteit Amsterdam develops high-end force and fluorescence microscope to interrogate the assembly and the enzymatic activity of biomolecular complexes at the single molecule level and with high spatiotemporal resolution. We have a keen interest in the dynamics of polymerase assembly/elongation and of helicase double-stranded nucleic acid unwinding. Specifically, we aim at understanding how the composition of the replication-transcription complex from, e.g. SARS-CoV-2, regulates viral RNA synthesis. Within the READDI-AC consortium, we work closely with the interdisciplinary team in Core C to reveal the mechanism of action of antiviral drugs targeting the viral polymerase and helicase of several RNA viruses of concern using our state-of-the-art single-molecule approaches.
